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Anti-Mouse ROBO3 Polyclonal Antibody (MA318014)

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Aperçu
Numéro de catalogueMA318014
Description
Anti-Mouse ROBO3 Polyclonal Antibody (MA318014) is a rabbit polyclonal antibody detecting ROBO3 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesMouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Mouse ROBO3 (Gly173-Val345).
Cible Roundabout-like protein 3, ROBO3, Roundabout homolog 3
Purification Purified by antigen affinity column.
Numéro d'accès Q9Z2I4
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Roundabout homolog 3 (ROBO3) is a ~149 kDa protein. Receptor involved in axon guidance during development. Acts as a multifunctional regulator of pathfinding that simultaneously mediates NELL2 repulsion, inhibits SLIT repulsion, and facilitates Netrin-1/NTN1 attraction. In spinal cord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1/ROBO2. Binding OF NELL2 to the receptor ROBO3 promotes oligomerization of ROBO3, resulting in the repulsion of commissural axons in the midline. ROBO3 also indirectly boosts axon attraction to NTN1 without interacting with NTN1 itself.

1. Sabatier, C. et al. (2004) Cell 117, 157-69. PMID: 15084255
2. Chen, Z. et al. (2008) Neuron 58, 325-32. PMID: 18466743
3. Zelina, P. et al. (2014) Neuron 84, 1258-72. PMID: 25433640
4. Zhuang, M. et al. (2019) Nucleic acids research 47, 4765-4777. PMID: 30843071
5. Pak, JS. et al. (2020) Nature communications 11, 1489. PMID: 32198364
Note For research use only.
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Formula
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Anti-Mouse ROBO3 Polyclonal Antibody

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