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Каталожный номерMA318014
Описание
Anti-Mouse ROBO3 Polyclonal Antibody (MA318014) is a rabbit polyclonal antibody detecting ROBO3 in ELISA, IHC, WB. Suitable for Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовMouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Mouse ROBO3 (Gly173-Val345).
Цель Roundabout-like protein 3, ROBO3, Roundabout homolog 3
Очистка Purified by antigen affinity column.
Номер доступа Q9Z2I4
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Roundabout homolog 3 (ROBO3) is a ~149 kDa protein. Receptor involved in axon guidance during development. Acts as a multifunctional regulator of pathfinding that simultaneously mediates NELL2 repulsion, inhibits SLIT repulsion, and facilitates Netrin-1/NTN1 attraction. In spinal cord development plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1/ROBO2. Binding OF NELL2 to the receptor ROBO3 promotes oligomerization of ROBO3, resulting in the repulsion of commissural axons in the midline. ROBO3 also indirectly boosts axon attraction to NTN1 without interacting with NTN1 itself.

1. Sabatier, C. et al. (2004) Cell 117, 157-69. PMID: 15084255
2. Chen, Z. et al. (2008) Neuron 58, 325-32. PMID: 18466743
3. Zelina, P. et al. (2014) Neuron 84, 1258-72. PMID: 25433640
4. Zhuang, M. et al. (2019) Nucleic acids research 47, 4765-4777. PMID: 30843071
5. Pak, JS. et al. (2020) Nature communications 11, 1489. PMID: 32198364
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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