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Anti-Mouse Sonic Hedgehog/SHH Recombinant Antibody (6D7) (MS826107)

Anti-Mouse Sonic Hedgehog/SHH Recombinant Antibody (6D7)
Anti-Mouse Sonic Hedgehog/SHH Recombinant Antibody (6D7)
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Especificación:
  • 50ug
  • 100ug
Número:
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  • Vista general

  • Imágenes

  • Referencias

  • Datasheet

  • SDS

Vista general
Número de catálogoMS826107
Reactividad de especiesHuman, Mouse
AplicacionesELISA, FCM
Especie huéspedRat
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clon6D7
Objetivo ShhNC, Sonic hedgehog protein, Shh N-terminal processed signaling domains, SHH, Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains, ShhN, ShhNp, HHG-1
Nivel de endotoxinas < 10 EU/mg
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso Q62226
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Sonic hedgehog protein (SHH) is a ~47 kDa protein. The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum.

1. Roelink, H. et al. (1995) Cell 81, 445-55. PMID: 7736596
2. Bumcrot, DA. et al. (1995) Molecular and cellular biology 15, 2294-303. PMID: 7891723
3. Porter, JA. et al. (1996) Science (New York, N.Y.) 274, 255-9. PMID: 8824192
4. Chen, X. et al. (2011) The Journal of cell biology 192, 825-38. PMID: 21357747
Nota For research use only.
Imágenes
  • Anti-Mouse Sonic Hedgehog/SHH Recombinant Antibody (6D7)

    SDS-PAGE

    SDS-PAGE for Anti-Mouse SHH Antibody (6D7)

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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