Anti-Mouse VEGFA Recombinant Antibody (G6) (MB941013)

Name: Anti-Mouse VEGFA Recombinant Antibody (G6)
Brand: abinScience
SKU: MB941013
Price: 110 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

MB941013

Reactivité des espèces

Human, Mouse, Rat, Rabbit

Applications

Blocking, ELISA, FCM

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a, kappa

ID de clone

Applications testées

WB, FCM

Cible

L-VEGF, N-VEGF, VEGF, VEGFA, VPF, Vascular endothelial growth factor A, long form, Vascular permeability factor

Niveau d'endotoxines

< 10 EU/mg

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q00731

RRID

Anti-Mouse VEGFA Antibody (G6) (abinScience Cat# MB941013, RRID:AB_3727197)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Vascular endothelial growth factor A, long form (VEGFA) is a ~43 kDa protein. Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA is the therapeutic target of bevacizumab (Avastin).

1. Katsman, M. et al. (2022) Cells 11. PMID: 35455969

Note

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Images

Bioactivity

Detects Mouse VEGFA in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Mouse VEGFA Antibody (G6)
Flow CytoMetry

Flow-cytometry using FITC anti-human VEGF antibody. U87 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human VEGF monoclonal antibody (Catalog: MB941013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-VEGFA/VEGF165 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human VEGFA/VEGF165 (Catalog No: HB941011)
Lane 2: negative control

Reférences

Fiche technique

Anti-Mouse VEGFA Recombinant Antibody (G6).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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