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Anti-MPK6 Polyclonal Antibody (PR006014)

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Aperçu
Numéro de cataloguePR006014
Description
Anti-MPK6 Polyclonal Antibody (PR006014) is a rabbit polyclonal antibody detecting MPK6 in ELISA, IHC, WB. Suitable for Nicotiana tabacum, Medicago sativa, Pisum sativum, and Capsicum annuum.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesArabidopsis thaliana (Mouse-ear cress), Nicotiana tabacum (Common tobacco), Oryza sativa (Rice)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant MPK6 (Asn35-Gln395).
Cible Mitogen-activated protein kinase 6, AtMPK6, MAP kinase 6, EC:2.7.11.24, MPK6
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q39026, Q40532, Q07176, A0A1S3Z5Y0, Q06060, Q9LKZ1, Q84UI5
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
WB 1:500-1:2000
IHC 1:50-1:500
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Mitogen-activated protein kinase 6 (MPK6) is a ~45 kDa protein. Mitogen-activated protein kinase (MAPK) which regulates abscisic acid (ABA) responses in a MAPKKK20-MKK5-MPK6 cascade involved in root growth (e.g. root cell division and elongation) and stomatal response. Involved in oxidative stress-mediated signaling cascade (such as ozone). Involved in the innate immune MAP kinase signaling cascade (MEKK1, MKK4/MKK5 and MPK3/MPK6) downstream of bacterial flagellin receptor FLS2. May be involved in hypersensitive response (HR)-mediated signaling cascade by modulating LIP5 phosphorylation and subsequent multivesicular bodies (MVBs) trafficking.

1. Li, K. et al. (2017) Plant physiology 173, 1391-1408. PMID: 27913741
2. Zhang, Y. et al. (2015) Developmental cell 33, 136-49. PMID: 25843888
3. Forzani, C. et al. (2011) The FEBS journal 278, 1126-36. PMID: 21276203
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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