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Anti-MRV-3 Sigma1/S1 Polyclonal Antibody (VK094044)

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Aperçu
Numéro de catalogueVK094044
Description
Anti-MRV-3 Sigma1/S1 Polyclonal Antibody (VK094044) is a rabbit polyclonal antibody detecting MRV-3 Sigma1/S1 in ELISA, IHC, WB. Suitable for Bacillus phage SF6 and Reovirus type 3.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesMammalian orthoreovirus 1 (MRV1)
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant MRV-3 Sigma1/S1 (Val170-Thr455).
Cible sigma-1 protein, S1
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Numéro d'accès P03528, P21270
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Outer capsid protein sigma-1 (Sigma1/S1) is a ~49 kDa protein. Fiber-like molecule of about 30 nm that attaches the virion to the host cell membrane by binding with initial low affinity to sialic acid containing proteins, followed by high-affinity bindingto the entry receptor F11R/JAM-A. The interaction of sigma-1 with F11R is required for NF-kB activation and apoptosis. Binding to both sialic acid and F11R is required to induce maximal levels of apoptosis.

1. Barton, ES. et al. (2001) Cell 104, 441-51. PMID: 11239401
2. Kirchner, E. et al. (2008) PLoS pathogens 4, e1000235. PMID: 19079583
3. Reiter, DM. et al. (2011) PLoS pathogens 7, e1002166. PMID: 21829363
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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