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Anti-MRV-3 Sigma3/S4 Polyclonal Antibody (VK094024)

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Número de catálogoVK094024
Descripción
Anti-MRV-3 Sigma3/S4 Polyclonal Antibody (VK094024) is a rabbit polyclonal antibody detecting MRV-3 Sigma3/S4 in ELISA, IHC, WB. Suitable for Reovirus type 1, Reovirus type 2, and Reovirus type 3.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesMammalian orthoreovirus 1 (MRV1)
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant MRV-3 Sigma3/S4 (Met1-Gly365).
Objetivo S4
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Purified by antigen affinity column.
Número de acceso P30211, P07939, P03527
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000,IHC:1:50-1:500,WB:1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Outer capsid protein sigma-3 (Sigma3/S4) is a ~41 kDa protein. Outer layer capsid protein that stimulates translation by blocking the activation of the dsRNA-dependent protein kinase EIF2AK2/PKR, thereby inhibiting the host interferon response. Sigma3 prevents the activation of EIF2AK2 by competing with the kinase for dsRNA-binding. During virus assembly, the inner layer proteins lambda-1 and the turret protein lambda-2 form the star core, followed by the stepwise attachment of sigma-2 that completes the core and mu1/sigma3 that complete the outer layer. Binds to the host neuronal receptor RTN4R for viral entry into host neural cells.

Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-MRV-3 Sigma3/S4 Polyclonal Antibody

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