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Anti-MYC Polyclonal Antibody (HF713014)

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Aperçu
Numéro de catalogueHF713014
Description
Anti-MYC Polyclonal Antibody (HF713014) is a rabbit polyclonal antibody detecting MYC in ELISA, IHC, WB. Suitable for Human, Mouse, Cat, Rat, and Bovine.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human MYC (Glu351-Ser437).
Cible Proto-oncogene c-Myc, BHLHE39, MYC, Myc proto-oncogene protein, bHLHe39, Transcription factor p64, Class E basic helix-loop-helix protein 39
Purification Purified by antigen affinity column.
Numéro d'accès P01106
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Myc proto-oncogene protein is a ~50 kDa protein. Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Activates the transcription of growth-related genes. Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis. Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells. Functions with TAF6L to activate target gene expression through RNA polymerase II pause release.

1. Shi, Y. et al. (2014) eLife 3, e02349. PMID: 24940000
2. Kim, JY. et al. (2015) The American journal of pathology 185, 2061-8. PMID: 25956029
3. David, CJ. et al. (2010) Nature 463, 364-8. PMID: 20010808
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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