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Anti-Mycobacterium tuberculosis katG Recombinant Antibody (SAA2165) (JN041013)

Anti-Mycobacterium tuberculosis katG Recombinant Antibody (SAA2165)
Anti-Mycobacterium tuberculosis katG Recombinant Antibody (SAA2165)
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  • 1mg
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Vista general
Número de catálogoJN041013
Reactividad de especiesMycobacterium tuberculosis
AplicacionesELISA, WB
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1
ID de clonSAA2165
Objetivo Catalase-peroxidase, CP, 1.11.1.21, Peroxidase/catalase, katG, Rv1908c, MTCY180.10
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P9WIE5
RRID Anti-Mycobacterium tuberculosis katG Antibody (SAA2165) (abinScience Cat# JN041013, RRID:AB_3729533)
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:2000-1:20000
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Catalase-peroxidase (katG) is a ~80 kDa protein. Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity, oxidizing various electron donors including NADP(H). Protects M.tuberculosis against toxic reactive oxygen species (ROS) including hydrogen peroxide as well as organic peroxides and thus contributes to its survival within host macrophages by countering the phagocyte oxidative burst. Also displays efficient peroxynitritase activity, which may help the bacterium to persist in macrophages.

1. Singh, R. et al. (2008) Archives of biochemistry and biophysics 471, 207-14. PMID: 18178143
2. Johnsson, K. et al. (1997) The Journal of biological chemistry 272, 2834-40. PMID: 9006925
3. Ng, VH. et al. (2004) Molecular microbiology 52, 1291-302. PMID: 15165233
4. Sherman, DR. et al. (1996) Science (New York, N.Y.) 272, 1641-3. PMID: 8658136
5. Wengenack, NL. et al. (1999) Biochemical and biophysical research communications 256, 485-7. PMID: 10080924
Nota For research use only.
Imágenes
  • Anti-Mycobacterium tuberculosis katG Recombinant Antibody (SAA2165)

    SDS-PAGE

    SDS-PAGE for Anti-Mycobacterium tuberculosis katG Recombinant Antibody (SAA2165)

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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