Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody (JN091014)

Name: Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody
Brand: abinScience
SKU: JN091014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

JN091014

Description

Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody (JN091014) is a rabbit polyclonal antibody detecting cards in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Mycoplasma pneumoniae

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Mycoplasma pneumoniae CARDS (Met1-Phe591).

Cible

ADP-ribosylating toxin CARDS, 2.4.2.-, ADP-ribosyltransferase CARDS, Community-Acquired Respiratory Distress Syndrome Toxin, CARDX TX, cards, MPN_372, MP464, Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae)

Purification

Purified by antigen affinity column.

Numéro d'accès

P75409

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

ADP-ribosylating toxin CARDS is a ~68 kDa protein. The main virulence factor for this bacteria, a mono-ADP-ribosylating toxin (mART), that transfers the ADP-ribosyl group from NAD(+) to multiple target proteins in vitro. Also elicits cytopathic effects in mammalian cells, such as disorganization and disruption of respiratory epithelial integrity in tracheal epithelium and vacuolization in the cytoplasm of CHO and HeLa cells as well as in mice and baboons. Treatment of mice or baboons with CARDS elicits a response that is consistent with human M.pneumoniae infections and mouse models of both infection and intoxication, suggesting that CARDS toxin is sufficient to cause prolonged inflammatory responses and airway dysfunction. Treatment of baboons with CARDS induces a number of cytokines; G-CSF (40 fold), IL-1Ra (10 fold), IL-6 and IL-8 (333 and 100 fold, respectively), MIP-1a (5 fold), and RANTES (9 fold). Treatment of mice gives a similar response.

1. Kannan, TR. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6724-9. PMID: 16617115

2. Kannan, TR. et al. (2014) Molecular microbiology 93, 568-81. PMID: 24948331

3. Bose, S. et al. (2014) mBio 5. PMID: 25538194

4. Ramasamy, K. et al. (2018) mBio 9. PMID: 29362229

5. Balasubramanian, S. et al. (2019) Cellular microbiology 21, e13032. PMID: 30977272

6. Ramasamy, K. et al. (2021) Scientific reports 11, 11571. PMID: 34078958

7. Hardy, RD. et al. (2009) PloS one 4, e7562. PMID: 19859545

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-0108 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Mycoplasma pneumoniae CARDS (Catalog No: JN091012)

Reférences

Fiche technique

Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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