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| カタログ番号 | JN091014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody (JN091014) is a rabbit polyclonal antibody detecting cards in ELISA, IHC, WB.
Highlights
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| 種反応性 | Mycoplasma pneumoniae | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Mycoplasma pneumoniae CARDS (Met1-Phe591). | ||||||||
| ターゲット | ADP-ribosylating toxin CARDS, 2.4.2.-, ADP-ribosyltransferase CARDS, Community-Acquired Respiratory Distress Syndrome Toxin, CARDX TX, cards, MPN_372, MP464, Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P75409 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | ADP-ribosylating toxin CARDS is a ~68 kDa protein. The main virulence factor for this bacteria, a mono-ADP-ribosylating toxin (mART), that transfers the ADP-ribosyl group from NAD(+) to multiple target proteins in vitro. Also elicits cytopathic effects in mammalian cells, such as disorganization and disruption of respiratory epithelial integrity in tracheal epithelium and vacuolization in the cytoplasm of CHO and HeLa cells as well as in mice and baboons. Treatment of mice or baboons with CARDS elicits a response that is consistent with human M.pneumoniae infections and mouse models of both infection and intoxication, suggesting that CARDS toxin is sufficient to cause prolonged inflammatory responses and airway dysfunction. Treatment of baboons with CARDS induces a number of cytokines; G-CSF (40 fold), IL-1Ra (10 fold), IL-6 and IL-8 (333 and 100 fold, respectively), MIP-1a (5 fold), and RANTES (9 fold). Treatment of mice gives a similar response. 1. Kannan, TR. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6724-9. PMID: 16617115 2. Kannan, TR. et al. (2014) Molecular microbiology 93, 568-81. PMID: 24948331 3. Bose, S. et al. (2014) mBio 5. PMID: 25538194 4. Ramasamy, K. et al. (2018) mBio 9. PMID: 29362229 5. Balasubramanian, S. et al. (2019) Cellular microbiology 21, e13032. PMID: 30977272 6. Ramasamy, K. et al. (2021) Scientific reports 11, 11571. PMID: 34078958 7. Hardy, RD. et al. (2009) PloS one 4, e7562. PMID: 19859545 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-0108 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Mycoplasma pneumoniae CARDS (Catalog No: JN091012)
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中国武漢市深敦寺路666号C棟、武漢、430206
