Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody (JN091014)

Name: Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody
Brand: abinScience
SKU: JN091014
Price: 168 USD
Availability: InStock

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Обзор

Каталожный номер

JN091014

Описание

Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody (JN091014) is a rabbit polyclonal antibody detecting cards in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Mycoplasma pneumoniae

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Mycoplasma pneumoniae CARDS (Met1-Phe591).

Цель

ADP-ribosylating toxin CARDS, 2.4.2.-, ADP-ribosyltransferase CARDS, Community-Acquired Respiratory Distress Syndrome Toxin, CARDX TX, cards, MPN_372, MP464, Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae)

Очистка

Purified by antigen affinity column.

Номер доступа

P75409

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

ADP-ribosylating toxin CARDS is a ~68 kDa protein. The main virulence factor for this bacteria, a mono-ADP-ribosylating toxin (mART), that transfers the ADP-ribosyl group from NAD(+) to multiple target proteins in vitro. Also elicits cytopathic effects in mammalian cells, such as disorganization and disruption of respiratory epithelial integrity in tracheal epithelium and vacuolization in the cytoplasm of CHO and HeLa cells as well as in mice and baboons. Treatment of mice or baboons with CARDS elicits a response that is consistent with human M.pneumoniae infections and mouse models of both infection and intoxication, suggesting that CARDS toxin is sufficient to cause prolonged inflammatory responses and airway dysfunction. Treatment of baboons with CARDS induces a number of cytokines; G-CSF (40 fold), IL-1Ra (10 fold), IL-6 and IL-8 (333 and 100 fold, respectively), MIP-1a (5 fold), and RANTES (9 fold). Treatment of mice gives a similar response.

1. Kannan, TR. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6724-9. PMID: 16617115

2. Kannan, TR. et al. (2014) Molecular microbiology 93, 568-81. PMID: 24948331

3. Bose, S. et al. (2014) mBio 5. PMID: 25538194

4. Ramasamy, K. et al. (2018) mBio 9. PMID: 29362229

5. Balasubramanian, S. et al. (2019) Cellular microbiology 21, e13032. PMID: 30977272

6. Ramasamy, K. et al. (2021) Scientific reports 11, 11571. PMID: 34078958

7. Hardy, RD. et al. (2009) PloS one 4, e7562. PMID: 19859545

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-0108 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Mycoplasma pneumoniae CARDS (Catalog No: JN091012)

Ссылки

Даташит

Anti-Mycoplasma pneumoniae CARDS Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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