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Anti-MYL9 Polyclonal Antibody (HB901014)

Anti-MYL9 Polyclonal Antibody
Anti-MYL9 Polyclonal Antibody
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Aperçu
Numéro de catalogueHB901014
Description
Anti-MYL9 Polyclonal Antibody (HB901014) is a rabbit polyclonal antibody detecting MYL9 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human MYL9 (Arg5-Leu163).
Cible Myosin regulatory light chain MRLC1, 20 kDa myosin light chain, Myosin regulatory light chain 2, smooth muscle isoform, MYRL2, MYL9, MLC-2C, LC20, Myosin regulatory light chain 9, MLC2, MRLC1, Myosin RLC, Myosin regulatory light polypeptide 9
Purification Purified by antigen affinity column.
Numéro d'accès P24844
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Myosin regulatory light polypeptide 9 (MYL9) is a ~19 kDa protein. Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation.

1. Iwasaki, T. et al. (2001) Cell structure and function 26, 677-83. PMID: 11942626
2. Kumar, CC. et al. (1989) Biochemistry 28, 4027-35. PMID: 2526655
Note For research use only.
Images
  • Anti-MYL9 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-MYL9 polyclonal antibody at 1μg/ml on various samples.
    Lane 1: C2C12 cell lysate
    Lane 2: Caco2 cell lysate
    Lane 3: Daudi cell lysate
    Lane 4: PC12 cell lysate
    Lane 5: mouse Lung
    Lane 6: Mouse colon lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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