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Anti-NAA10 Polyclonal Antibody (HA630014)

Anti-NAA10 Polyclonal Antibody
Anti-NAA10 Polyclonal Antibody
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Vista general
Número de catálogoHA630014
Descripción
Anti-NAA10 Polyclonal Antibody (HA630014) is a rabbit polyclonal antibody detecting N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human NAA10 (Met1-Ser235).
Objetivo N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P41227, Q2KI14, Q9QY36
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

N-alpha-acetyltransferase 10 (NAA10) is a ~26 kDa protein. Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity. Acetylates amino termini that are devoid of initiator methionine. The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration.

1. Arnesen, T. et al. (2005) The Biochemical journal 386, 433-43. PMID: 15496142
2. Arnesen, T. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 8157-62. PMID: 19420222
3. Shin, DH. et al. (2009) PloS one 4, e7451. PMID: 19826488
4. Kuo, HP. et al. (2010) Science signaling 3, ra9. PMID: 20145209
5. Arnesen, T. et al. (2010) Molecular and cellular biology 30, 1898-909. PMID: 20154145
6. Myklebust, LM. et al. (2015) Human molecular genetics 24, 1956-76. PMID: 25489052
7. Seo, JH. et al. (2016) Nature communications 7, 12882. PMID: 27708256
8. Gottlieb, L. et al. (2018) Structure (London, England : 1993) 26, 925-935.e8. PMID: 29754825
9. Deng, S. et al. (2020) Nature communications 11, 818. PMID: 32042062
10. Jeong, JW. et al. (2002) Cell 111, 709-20. PMID: 12464182
Nota For research use only
Imágenes
  • Anti-NAA10 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NAA10 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: 293T cell lysate
    Lane 2: Hela cell lysate
    Lane 3: MCF-7 cell lysate
    Lane 4: HT-29 cell lysate
    Lane 5: C2C12 cell lysate
    Lane 6: H1299 cell lysate

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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