Anti-NAA10 Polyclonal Antibody (HA630014)

Name: Anti-NAA10 Polyclonal Antibody
Brand: abinScience
SKU: HA630014
Price: 96 USD
Availability: InStock

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50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HA630014

Description

Anti-NAA10 Polyclonal Antibody (HA630014) is a rabbit polyclonal antibody detecting N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human NAA10 (Met1-Ser235).

Cible

N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

P41227, Q2KI14, Q9QY36

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

N-alpha-acetyltransferase 10 (NAA10) is a ~26 kDa protein. Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity. Acetylates amino termini that are devoid of initiator methionine. The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration.

1. Arnesen, T. et al. (2005) The Biochemical journal 386, 433-43. PMID: 15496142

2. Arnesen, T. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 8157-62. PMID: 19420222

3. Shin, DH. et al. (2009) PloS one 4, e7451. PMID: 19826488

4. Kuo, HP. et al. (2010) Science signaling 3, ra9. PMID: 20145209

5. Arnesen, T. et al. (2010) Molecular and cellular biology 30, 1898-909. PMID: 20154145

6. Myklebust, LM. et al. (2015) Human molecular genetics 24, 1956-76. PMID: 25489052

7. Seo, JH. et al. (2016) Nature communications 7, 12882. PMID: 27708256

8. Gottlieb, L. et al. (2018) Structure (London, England : 1993) 26, 925-935.e8. PMID: 29754825

9. Deng, S. et al. (2020) Nature communications 11, 818. PMID: 32042062

10. Jeong, JW. et al. (2002) Cell 111, 709-20. PMID: 12464182

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-NAA10 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: HT-29 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: H1299 cell lysate

Reférences

Fiche technique

Anti-NAA10 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-NAA10 Polyclonal Antibody (HA630014)

Aperçu

Images

Reférences

Fiche technique

SDS