Anti-NAA10 Polyclonal Antibody (HA630014)

Name: Anti-NAA10 Polyclonal Antibody
Brand: abinScience
SKU: HA630014
Price: 96 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HA630014

説明

Anti-NAA10 Polyclonal Antibody (HA630014) is a rabbit polyclonal antibody detecting N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant human NAA10 (Met1-Ser235).

ターゲット

N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2

エンドトキシンレベル

Please contact with the lab for this information.

精製

Purified by antigen affinity column.

アクセッション番号

P41227, Q2KI14, Q9QY36

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

N-alpha-acetyltransferase 10 (NAA10) is a ~26 kDa protein. Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity. Acetylates amino termini that are devoid of initiator methionine. The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration.

1. Arnesen, T. et al. (2005) The Biochemical journal 386, 433-43. PMID: 15496142

2. Arnesen, T. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 8157-62. PMID: 19420222

3. Shin, DH. et al. (2009) PloS one 4, e7451. PMID: 19826488

4. Kuo, HP. et al. (2010) Science signaling 3, ra9. PMID: 20145209

5. Arnesen, T. et al. (2010) Molecular and cellular biology 30, 1898-909. PMID: 20154145

6. Myklebust, LM. et al. (2015) Human molecular genetics 24, 1956-76. PMID: 25489052

7. Seo, JH. et al. (2016) Nature communications 7, 12882. PMID: 27708256

8. Gottlieb, L. et al. (2018) Structure (London, England : 1993) 26, 925-935.e8. PMID: 29754825

9. Deng, S. et al. (2020) Nature communications 11, 818. PMID: 32042062

10. Jeong, JW. et al. (2002) Cell 111, 709-20. PMID: 12464182

注意事項

For research use only

イメージ

Western blot

Western blot analysis was performed using anti-NAA10 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: HT-29 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: H1299 cell lysate

参考文献

データシート

Anti-NAA10 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-NAA10 Polyclonal Antibody (HA630014)

概要

イメージ

参考文献

データシート

SDS