Anti-NAA10 Polyclonal Antibody (HA630014)

Name: Anti-NAA10 Polyclonal Antibody
Brand: abinScience
SKU: HA630014
Price: 96 USD
Availability: InStock

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Каталожный номер

HA630014

Описание

Anti-NAA10 Polyclonal Antibody (HA630014) is a rabbit polyclonal antibody detecting N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2 in ELISA, IHC, WB. Suitable for Human, Bovine, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant human NAA10 (Met1-Ser235).

Цель

N-alpha-acetyltransferase 10; 2.3.1.255; N-terminal acetyltransferase complex ARD1 subunit homolog A; hARD1; NatA catalytic subunit Naa10; NAA10; ARD1; ARD1A; TE2

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

P41227, Q2KI14, Q9QY36

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

N-alpha-acetyltransferase 10 (NAA10) is a ~26 kDa protein. Catalytic subunit of N-terminal acetyltransferase complexes which display alpha (N-terminal) acetyltransferase activity. Acetylates amino termini that are devoid of initiator methionine. The alpha (N-terminal) acetyltransferase activity may be important for vascular, hematopoietic and neuronal growth and development. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. Represses MYLK kinase activity by acetylation, and thus represses tumor cell migration.

1. Arnesen, T. et al. (2005) The Biochemical journal 386, 433-43. PMID: 15496142

2. Arnesen, T. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 8157-62. PMID: 19420222

3. Shin, DH. et al. (2009) PloS one 4, e7451. PMID: 19826488

4. Kuo, HP. et al. (2010) Science signaling 3, ra9. PMID: 20145209

5. Arnesen, T. et al. (2010) Molecular and cellular biology 30, 1898-909. PMID: 20154145

6. Myklebust, LM. et al. (2015) Human molecular genetics 24, 1956-76. PMID: 25489052

7. Seo, JH. et al. (2016) Nature communications 7, 12882. PMID: 27708256

8. Gottlieb, L. et al. (2018) Structure (London, England : 1993) 26, 925-935.e8. PMID: 29754825

9. Deng, S. et al. (2020) Nature communications 11, 818. PMID: 32042062

10. Jeong, JW. et al. (2002) Cell 111, 709-20. PMID: 12464182

Примечание

For research use only

Изображения

Western blot

Western blot analysis was performed using anti-NAA10 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Hela cell lysate
Lane 3: MCF-7 cell lysate
Lane 4: HT-29 cell lysate
Lane 5: C2C12 cell lysate
Lane 6: H1299 cell lysate

Ссылки

Даташит

Anti-NAA10 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-NAA10 Polyclonal Antibody (HA630014)

Обзор

Изображения

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Даташит

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