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Anti-NARS1 Polyclonal Antibody (HT196014)

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Anti-NARS1 Polyclonal Antibody
Anti-NARS1 Polyclonal Antibody
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Aperçu
Numéro de catalogueHT196014
Description
Anti-NARS1 Polyclonal Antibody (HT196014) is a rabbit polyclonal antibody detecting NARS1 in ELISA, IHC, WB. Suitable for Human, Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human NARS1 (Met1-Arg330).
Cible Asparagine--tRNA ligase, cytoplasmic, NARS, NRS, Asparaginyl-tRNA synthetase, NARS1, Asparaginyl-tRNA synthetase 1, AsnRS
Purification Purified by antigen affinity column.
Numéro d'accès O43776
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Asparagine--tRNA ligase, cytoplasmic (NARS1) is a ~62 kDa protein. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. Has an essential role in the development of the cerebral cortex, being required for proper proliferation of radial glial cells.

1. Manole, A. et al. (2020) American journal of human genetics 107, 311-324. PMID: 32738225
2. Wang, L. et al. (2020) Nature communications 11, 4038. PMID: 32788587
3. Beaulande, M. et al. (1998) Nucleic acids research 26, 521-4. PMID: 9421509
4. Howard, OM. et al. (2002) The Journal of experimental medicine 196, 781-91. PMID: 12235211
5. Park, JS. et al. (2018) International journal of biological macromolecules 120, 835-845. PMID: 30171954
Note For research use only.
Images
  • Anti-NARS1 Polyclonal Antibody

    Western blot

    Lane 1: HepG2 cell lysate
    Lane 2: K562 cell lysate
    Lane 3: Hela cell lysate
    Lane 4: RAW264.7 cell lysate
    Lane 5: 293T cell lysate
    Lane 6: LO2 cell lysate
    Lane 7: Jurkat cell lysate

Reférences
Fiche technique
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-NARS1 Polyclonal Antibody

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