Anti-NARS1 Polyclonal Antibody (HT196014)

Name: Anti-NARS1 Polyclonal Antibody
Brand: abinScience
SKU: HT196014
Price: 96 USD
Availability: InStock

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Каталожный номер

HT196014

Описание

Anti-NARS1 Polyclonal Antibody (HT196014) is a rabbit polyclonal antibody detecting NARS1 in ELISA, IHC, WB. Suitable for Human, Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human NARS1 (Met1-Arg330).

Цель

Asparagine--tRNA ligase, cytoplasmic, NARS, NRS, Asparaginyl-tRNA synthetase, NARS1, Asparaginyl-tRNA synthetase 1, AsnRS

Очистка

Purified by antigen affinity column.

Номер доступа

O43776

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Asparagine--tRNA ligase, cytoplasmic (NARS1) is a ~62 kDa protein. Catalyzes the attachment of asparagine to tRNA(Asn) in a two-step reaction: asparagine is first activated by ATP to form Asn-AMP and then transferred to the acceptor end of tRNA(Asn). In addition to its essential role in protein synthesis, acts as a signaling molecule that induced migration of CCR3-expressing cells. Has an essential role in the development of the cerebral cortex, being required for proper proliferation of radial glial cells.

1. Manole, A. et al. (2020) American journal of human genetics 107, 311-324. PMID: 32738225

2. Wang, L. et al. (2020) Nature communications 11, 4038. PMID: 32788587

3. Beaulande, M. et al. (1998) Nucleic acids research 26, 521-4. PMID: 9421509

4. Howard, OM. et al. (2002) The Journal of experimental medicine 196, 781-91. PMID: 12235211

5. Park, JS. et al. (2018) International journal of biological macromolecules 120, 835-845. PMID: 30171954

Примечание

For research use only.

Изображения

Western blot

Lane 1: HepG2 cell lysate
Lane 2: K562 cell lysate
Lane 3: Hela cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: 293T cell lysate
Lane 6: LO2 cell lysate
Lane 7: Jurkat cell lysate

Ссылки

Даташит

Anti-NARS1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-NARS1 Polyclonal Antibody (HT196014)

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Изображения

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