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Anti-NCF1 Polyclonal Antibody (HB843014)

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Aperçu
Numéro de catalogueHB843014
Description
Anti-NCF1 Polyclonal Antibody (HB843014) is a rabbit polyclonal antibody detecting NCF1 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human NCF1 (Met1-Val390).
Cible SH3PXD1A, NCF1, p47-phox, 47 kDa autosomal chronic granulomatous disease protein, Nox-organizing protein 2, NOXO2, SH3 and PX domain-containing protein 1A, NCF-1, 47 kDa neutrophil oxidase factor, NCF-47K, Nox organizer 2, Neutrophil NADPH oxidase factor 1, Neutrophil cytosol factor 1
Purification Purified by antigen affinity column.
Numéro d'accès P14598
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Neutrophil cytosol factor 1 (NCF1) is a ~44 kDa protein. Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction. Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane. This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox.

1. Lomax, KJ. et al. (1989) Science (New York, N.Y.) 245, 409-12. PMID: 2547247
2. Volpp, BD. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 7195-9. PMID: 2550933
3. Liu, X. et al. (2024) Nature 627, 189-195. PMID: 38355798
4. Groemping, Y. et al. (2003) Cell 113, 343-55. PMID: 12732142
5. Kilpatrick, LE. et al. (2010) Journal of leukocyte biology 87, 153-64. PMID: 19801500
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Volume
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-NCF1 Polyclonal Antibody

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