Anti-NCOA2 Polyclonal Antibody (HA816014)

Name: Anti-NCOA2 Polyclonal Antibody
Brand: abinScience
SKU: HA816014
Price: 96 USD
Availability: InStock

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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HA816014

Description

Anti-NCOA2 Polyclonal Antibody (HA816014) is a rabbit polyclonal antibody detecting Nuclear receptor coactivator 2; NCoA-2; Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2; NCOA2; BHLHE75; SRC2; TIF2 in ELISA, IHC, WB. Suitable for Human, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human NCOA2 (Val109-Cys380).

Cible

Nuclear receptor coactivator 2; NCoA-2; Class E basic helix-loop-helix protein 75; bHLHe75; Transcriptional intermediary factor 2; hTIF2; NCOA2; BHLHE75; SRC2; TIF2

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q15596, Q9WUI9, Q61026

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Nuclear receptor coactivator 2 (NCOA2) is a ~159 kDa protein. Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. Critical regulator of glucose metabolism regulation, acts as a RORA coactivator to specifically modulate G6PC1 expression. Involved in the positive regulation of the transcriptional activity of the glucocorticoid receptor NR3C1 by sumoylation enhancer RWDD3.

1. Druker, J. et al. (2013) Molecular and cellular biology 33, 2116-27. PMID: 23508108

2. Voegel, JJ. et al. (1996) The EMBO journal 15, 3667-75. PMID: 8670870

3. Voegel, JJ. et al. (1998) The EMBO journal 17, 507-19. PMID: 9430642

4. Musille, PM. et al. (2012) Nature structural & molecular biology 19, 532-S2. PMID: 22504882

5. Musille, PM. et al. (2016) The Journal of biological chemistry 291, 1411-26. PMID: 26553876

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-NCOA2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: Jurkat cell lysate

Reférences

Fiche technique

Anti-NCOA2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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