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Anti-NCSTN Polyclonal Antibody (HV481014)

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Vista general
Número de catálogoHV481014
Descripción
Anti-NCSTN Polyclonal Antibody (HV481014) is a rabbit polyclonal antibody detecting NCSTN in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human NCSTN (Gln420-Asp655).
Objetivo KIAA0253, Nicastrin, NCSTN
Purificación Purified by antigen affinity column.
Número de acceso Q92542
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Nicastrin (NCSTN) is a ~78 kDa protein. Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.

1. Yu, G. et al. (2000) Nature 407, 48-54. PMID: 10993067
2. Edbauer, D. et al. (2003) Nature cell biology 5, 486-8. PMID: 12679784
3. Lu, P. et al. (2014) Nature 512, 166-170. PMID: 25043039
4. Bai, XC. et al. (2015) Nature 525, 212-217. PMID: 26280335
5. Yang, G. et al. (2019) Nature 565, 192-197. PMID: 30598546
6. Zhou, R. et al. (2019) Science (New York, N.Y.) 363. PMID: 30630874
Nota For research use only.
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Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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