Anti-NDC80/HEC1 Polyclonal Antibody (HT177014)

Name: Anti-NDC80/HEC1 Polyclonal Antibody
Brand: abinScience
SKU: HT177014
Price: 168 USD
Availability: InStock

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Каталожный номер

HT177014

Описание

Anti-NDC80/HEC1 Polyclonal Antibody (HT177014) is a rabbit polyclonal antibody detecting NDC80/HEC1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human NDC80/HEC1 (Pro290-Glu642).

Цель

HEC1, Kinetochore protein Hec1, HsHec1, HEC, Kinetochore protein NDC80 homolog, Kinetochore-associated protein 2, NDC80, Highly expressed in cancer protein, Retinoblastoma-associated protein HEC, KNTC2

Очистка

Purified by antigen affinity column.

Номер доступа

O14777

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Kinetochore protein NDC80 homolog (HEC1) is a ~73 kDa protein. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.

1. Martin-Lluesma, S. et al. (2002) Science (New York, N.Y.) 297, 2267-70. PMID: 12351790

2. DeLuca, JG. et al. (2003) Current biology : CB 13, 2103-9. PMID: 14654001

3. Bharadwaj, R. et al. (2004) The Journal of biological chemistry 279, 13076-85. PMID: 14699129

4. Joseph, J. et al. (2004) Current biology : CB 14, 611-7. PMID: 15062103

5. Stucke, VM. et al. (2004) Chromosoma 113, 1-15. PMID: 15235793

6. Meraldi, P. et al. (2004) Developmental cell 7, 45-60. PMID: 15239953

7. DeLuca, JG. et al. (2005) Molecular biology of the cell 16, 519-31. PMID: 15548592

8. Lin, YT. et al. (2006) Oncogene 25, 6901-14. PMID: 16732327

9. Zhao, G. et al. (2019) The Journal of biological chemistry 294, 576-592. PMID: 30409912

10. Chen, Y. et al. (1997) Molecular and cellular biology 17, 6049-56. PMID: 9315664

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-NDC80/HEC1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-NDC80/HEC1 Polyclonal Antibody (HT177014)

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