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Anti-NDUFS1 Polyclonal Antibody (HB624014)

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概要
カタログ番号HB624014
説明
Anti-NDUFS1 Polyclonal Antibody (HB624014) is a rabbit polyclonal antibody detecting NDUFS1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Macaca fascicularis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human, Mouse, Rat
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human NDUFS1 (Ala524-Cys727).
ターゲット Complex I-75kD, CI-75kD, NDUFS1, NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial
精製 Purified by antigen affinity column.
アクセッション番号 P28331
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1) is a ~79 kDa protein. Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which catalyzes electron transfer from NADH through the respiratory chain, using ubiquinone as an electron acceptor. Essential for catalysing the entry and efficient transfer of electrons within complex I. Plays a key role in the assembly and stability of complex I and participates in the association of complex I with ubiquinol-cytochrome reductase complex (Complex III) to form supercomplexes.

1. Elkholi, R. et al. (2019) Molecular cell 74, 452-465.e7. PMID: 30879903
2. Ni, Y. et al. (2019) Cells 8. PMID: 31557978
注意事項 For research use only.
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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