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Anti-NEFL Polyclonal Antibody (HY123014)

Anti-NEFL Polyclonal Antibody
Anti-NEFL Polyclonal Antibody
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概要
カタログ番号HY123014
説明
Anti-NEFL Polyclonal Antibody (HY123014) is a rabbit polyclonal antibody detecting NEFL in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human, Mouse, Rat
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human NEFL (Met1-Asn352).
ターゲット NEFL, Neurofilament light polypeptide, 68 kDa neurofilament protein, NF68, Neurofilament triplet L protein, NF-L, NFL
精製 Purified by antigen affinity column.
アクセッション番号 P07196
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Neurofilament light polypeptide (NEFL) is a ~61 kDa protein. Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.

注意事項 For research use only.
イメージ
  • Anti-NEFL Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-NEFL polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Mouse brain lysate
    Lane 2: Mouse cerebellum lysate
    Lane 3: Rat brain lysate
    Lane 4: Rat cerebellum lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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