Anti-IkB-alpha Polyclonal Antibody (HB155014)

Name: Anti-IkB-alpha Polyclonal Antibody
Brand: abinScience
SKU: HB155014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
50ug
100ug

Número:

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Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HB155014

Descripción

Anti-NFKBIA Polyclonal Antibody (HB155014) is a rabbit polyclonal antibody detecting NFKBIA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human NFKBIA (Lys67-Glu302).

Objetivo

I-kappa-B-alpha, IKBA, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

P25963

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Envío

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

NF-kappa-B inhibitor alpha (NFKBIA) is a ~35 kDa protein. Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

1. Ganchi, PA. et al. (1992) Molecular biology of the cell 3, 1339-52. PMID: 1493333

2. Quan, J. et al. (2023) Journal of immunology (Baltimore, Md. : 1950) 210, 640-652. PMID: 36651806

3. Scherer, DC. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 11259-63. PMID: 7479976

4. Chen, Z. et al. (1995) Genes & development 9, 1586-97. PMID: 7628694

5. Traenckner, EB. et al. (1995) The EMBO journal 14, 2876-83. PMID: 7796813

6. Brown, K. et al. (1995) Science (New York, N.Y.) 267, 1485-8. PMID: 7878466

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-NFKBIA polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate

Referencias

Datasheet

Anti-IkB-alpha Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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