Anti-IkB-alpha Polyclonal Antibody (HB155014)

Name: Anti-IkB-alpha Polyclonal Antibody
Brand: abinScience
SKU: HB155014
Price: 96 USD
Availability: InStock

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Даташит
SDS

Обзор

Каталожный номер

HB155014

Описание

Anti-NFKBIA Polyclonal Antibody (HB155014) is a rabbit polyclonal antibody detecting NFKBIA in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human NFKBIA (Lys67-Glu302).

Цель

I-kappa-B-alpha, IKBA, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

P25963

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Доставка

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

NF-kappa-B inhibitor alpha (NFKBIA) is a ~35 kDa protein. Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals. On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

1. Ganchi, PA. et al. (1992) Molecular biology of the cell 3, 1339-52. PMID: 1493333

2. Quan, J. et al. (2023) Journal of immunology (Baltimore, Md. : 1950) 210, 640-652. PMID: 36651806

3. Scherer, DC. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 11259-63. PMID: 7479976

4. Chen, Z. et al. (1995) Genes & development 9, 1586-97. PMID: 7628694

5. Traenckner, EB. et al. (1995) The EMBO journal 14, 2876-83. PMID: 7796813

6. Brown, K. et al. (1995) Science (New York, N.Y.) 267, 1485-8. PMID: 7878466

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-NFKBIA polyclonal antibody at 1ug/mL on various samples.
Lane 1: NIH3T3 cell lysate

Ссылки

Даташит

Anti-IkB-alpha Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа