Anti-NGB Polyclonal Antibody (HV356014)

Name: Anti-NGB Polyclonal Antibody
Brand: abinScience
SKU: HV356014
Price: 168 USD
Availability: InStock

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Especificación:

50ug
100ug

Número:

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HV356014

Descripción

Anti-NGB Polyclonal Antibody (HV356014) is a rabbit polyclonal antibody detecting NGB in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human NGB (Met1-Glu151).

Objetivo

NGB, Neuroglobin

Purificación

Purified by antigen affinity column.

Número de acceso

Q9NPG2

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Neuroglobin (NGB) is a ~16 kDa protein. Monomeric globin with a bis-histidyl six-coordinate heme-iron atom through which it can bind dioxygen, carbon monoxide and nitric oxide. Could help transport oxygen and increase its availability to the metabolically active neuronal tissues, though its low quantity in tissues as well as its high affinity for dioxygen, which may limit its oxygen-releasing ability, argue against it. The ferrous/deoxygenated form exhibits a nitrite reductase activity and it could produce nitric oxide which in turn inhibits cellular respiration in response to hypoxia. In its ferrous/deoxygenated state, it may also exhibit GDI (Guanine nucleotide Dissociation Inhibitor) activity toward heterotrimeric G-alpha proteins, thereby regulating signal transduction to facilitate neuroprotective responses in the wake of hypoxia and associated oxidative stress.

1. Dewilde, S. et al. (2001) The Journal of biological chemistry 276, 38949-55. PMID: 11473128

2. Pesce, A. et al. (2003) Structure (London, England : 1993) 11, 1087-95. PMID: 12962627

3. Guimarães, BG. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1005-14. PMID: 24699645

4. Wakasugi, K. et al. (2003) The Journal of biological chemistry 278, 36505-12. PMID: 12860983

5. Tiso, M. et al. (2011) The Journal of biological chemistry 286, 18277-89. PMID: 21296891

6. Watanabe, S. et al. (2008) Biochemical and biophysical research communications 369, 695-700. PMID: 18302932

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-NGB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo