Anti-NGB Polyclonal Antibody (HV356014)

Name: Anti-NGB Polyclonal Antibody
Brand: abinScience
SKU: HV356014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HV356014

Description

Anti-NGB Polyclonal Antibody (HV356014) is a rabbit polyclonal antibody detecting NGB in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human NGB (Met1-Glu151).

Cible

NGB, Neuroglobin

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9NPG2

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Neuroglobin (NGB) is a ~16 kDa protein. Monomeric globin with a bis-histidyl six-coordinate heme-iron atom through which it can bind dioxygen, carbon monoxide and nitric oxide. Could help transport oxygen and increase its availability to the metabolically active neuronal tissues, though its low quantity in tissues as well as its high affinity for dioxygen, which may limit its oxygen-releasing ability, argue against it. The ferrous/deoxygenated form exhibits a nitrite reductase activity and it could produce nitric oxide which in turn inhibits cellular respiration in response to hypoxia. In its ferrous/deoxygenated state, it may also exhibit GDI (Guanine nucleotide Dissociation Inhibitor) activity toward heterotrimeric G-alpha proteins, thereby regulating signal transduction to facilitate neuroprotective responses in the wake of hypoxia and associated oxidative stress.

1. Dewilde, S. et al. (2001) The Journal of biological chemistry 276, 38949-55. PMID: 11473128

2. Pesce, A. et al. (2003) Structure (London, England : 1993) 11, 1087-95. PMID: 12962627

3. Guimarães, BG. et al. (2014) Acta crystallographica. Section D, Biological crystallography 70, 1005-14. PMID: 24699645

4. Wakasugi, K. et al. (2003) The Journal of biological chemistry 278, 36505-12. PMID: 12860983

5. Tiso, M. et al. (2011) The Journal of biological chemistry 286, 18277-89. PMID: 21296891

6. Watanabe, S. et al. (2008) Biochemical and biophysical research communications 369, 695-700. PMID: 18302932

Note

For research use only.

Images

Reférences

Fiche technique

Anti-NGB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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