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Обзор
Каталожный номерHX241014
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human NME5 (Ile3-Lys194).
Цель nm23-H5, Testis-specific nm23 homolog, NDK-H 5, Inhibitor of p53-induced apoptosis-beta, NME5, NDP kinase homolog 5, IPIA-beta, Nucleoside diphosphate kinase homolog 5
Очистка Purified by antigen affinity column.
Номер доступа P56597
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Nucleoside diphosphate kinase 5 (NME5) is a ~24 kDa protein. Functions as part of axonemal radial spoke complexes, which play an important part in the motility of sperm and cilia. In vitro, does not display nucleoside diphosphate kinase (NDPK) activity (Probable). Exhibits a 3'-5'-DNA exonuclease activity that removes single nucleotides from the 3' terminus of single-stranded DNA substrates and digests overhanging mismatched 3' termini from double-stranded DNA substrates, possibly participating in DNA nucleolytic processing. Confers protection from cell death mediated by BAX and alters the cellular levels of several antioxidant enzymes, including GPX5. Might be involved in spermiogenesis by enhancing the ability of late-stage spermatids to eliminate reactive oxygen species.

1. Yoon, JH. et al. (2005) Biochemistry 44, 15774-86. PMID: 16313181
2. Munier, A. et al. (1998) FEBS letters 434, 289-94. PMID: 9742940
Примечание For research use only.
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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