Anti-NME6 Polyclonal Antibody (HF556014)

Name: Anti-NME6 Polyclonal Antibody
Brand: abinScience
SKU: HF556014
Price: 96 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) :

Spécification :

20ug
50ug
100ug

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HF556014

Description

Anti-NME6 Polyclonal Antibody (HF556014) is a rabbit polyclonal antibody detecting NME6 in ELISA, IHC, WB. Suitable for Mouse, Rat, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human NME6 (Leu11-Ala186).

Cible

nm23-H6, Nucleoside diphosphate kinase 6, IPIA-alpha, NME6, NDP kinase 6, Inhibitor of p53-induced apoptosis-alpha, NDK 6

Purification

Purified by antigen affinity column.

Numéro d'accès

O75414

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Nucleoside diphosphate kinase 6, mitochondrial (NME6) is a ~21 kDa protein. Catalytic subunit of a complex with RCC1L, which catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving the formation of a phosphohistidine intermediate. Participates in the regulation of mitochondrial nucleotide levels, mitoribosome biogenesis, and mitochondrial RNA abundances. Supplies pyrimidine ribonucleoside triphosphates (rNTPs) for mitochondrial transcription and is required for the maintenance of mitochondrial DNA (mtDNA) when the import of pyrimidine deoxyribonucleoside triphosphates (dNTPs) is limited from the cytosol is limited. As a monomer, lacks nucleoside diphosphate kinase (NDPK) activity.

1. Tsuiki, H. et al. (1999) Journal of cellular biochemistry 76, 254-69. PMID: 10618642

2. Proust, B. et al. (2021) Cell & bioscience 11, 195. PMID: 34789336

3. Kramer, NJ. et al. (2023) Nature cell biology 25, 1575-1589. PMID: 37770567

4. Grotehans, N. et al. (2023) The EMBO journal 42, e113256. PMID: 37439264

Note

For research use only.

Images

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting NME6 (HF556014) at a concentration of 26.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of NME6 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-NME6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Lncap cell lysate
Lane 2: U20S cell lysate

Reférences

Fiche technique

Anti-NME6 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai