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| カタログ番号 | HF556014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-NME6 Polyclonal Antibody (HF556014) is a rabbit polyclonal antibody detecting NME6 in ELISA, IHC, WB. Suitable for Mouse, Rat, and Human.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human NME6 (Leu11-Ala186). | ||||||||
| ターゲット | nm23-H6, Nucleoside diphosphate kinase 6, IPIA-alpha, NME6, NDP kinase 6, Inhibitor of p53-induced apoptosis-alpha, NDK 6 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O75414 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Nucleoside diphosphate kinase 6, mitochondrial (NME6) is a ~21 kDa protein. Catalytic subunit of a complex with RCC1L, which catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving the formation of a phosphohistidine intermediate. Participates in the regulation of mitochondrial nucleotide levels, mitoribosome biogenesis, and mitochondrial RNA abundances. Supplies pyrimidine ribonucleoside triphosphates (rNTPs) for mitochondrial transcription and is required for the maintenance of mitochondrial DNA (mtDNA) when the import of pyrimidine deoxyribonucleoside triphosphates (dNTPs) is limited from the cytosol is limited. As a monomer, lacks nucleoside diphosphate kinase (NDPK) activity. 1. Tsuiki, H. et al. (1999) Journal of cellular biochemistry 76, 254-69. PMID: 10618642 2. Proust, B. et al. (2021) Cell & bioscience 11, 195. PMID: 34789336 3. Kramer, NJ. et al. (2023) Nature cell biology 25, 1575-1589. PMID: 37770567 4. Grotehans, N. et al. (2023) The EMBO journal 42, e113256. PMID: 37439264 | ||||||||
| 注意事項 | For research use only. |
Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting NME6 (HF556014) at a concentration of 26.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of NME6 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot analysis was performed using anti-NME6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Lncap cell lysate
Lane 2: U20S cell lysate
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