Anti-NME6 Polyclonal Antibody (HF556014)

Name: Anti-NME6 Polyclonal Antibody
Brand: abinScience
SKU: HF556014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HF556014

Описание

Anti-NME6 Polyclonal Antibody (HF556014) is a rabbit polyclonal antibody detecting NME6 in ELISA, IHC, WB. Suitable for Mouse, Rat, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human NME6 (Leu11-Ala186).

Цель

nm23-H6, Nucleoside diphosphate kinase 6, IPIA-alpha, NME6, NDP kinase 6, Inhibitor of p53-induced apoptosis-alpha, NDK 6

Очистка

Purified by antigen affinity column.

Номер доступа

O75414

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Nucleoside diphosphate kinase 6, mitochondrial (NME6) is a ~21 kDa protein. Catalytic subunit of a complex with RCC1L, which catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving the formation of a phosphohistidine intermediate. Participates in the regulation of mitochondrial nucleotide levels, mitoribosome biogenesis, and mitochondrial RNA abundances. Supplies pyrimidine ribonucleoside triphosphates (rNTPs) for mitochondrial transcription and is required for the maintenance of mitochondrial DNA (mtDNA) when the import of pyrimidine deoxyribonucleoside triphosphates (dNTPs) is limited from the cytosol is limited. As a monomer, lacks nucleoside diphosphate kinase (NDPK) activity.

1. Tsuiki, H. et al. (1999) Journal of cellular biochemistry 76, 254-69. PMID: 10618642

2. Proust, B. et al. (2021) Cell & bioscience 11, 195. PMID: 34789336

3. Kramer, NJ. et al. (2023) Nature cell biology 25, 1575-1589. PMID: 37770567

4. Grotehans, N. et al. (2023) The EMBO journal 42, e113256. PMID: 37439264

Примечание

For research use only.

Изображения

Immunofluorescence

Hela cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting NME6 (HF556014) at a concentration of 26.6 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of NME6 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).
Western blot

Western blot analysis was performed using anti-NME6 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Lncap cell lysate
Lane 2: U20S cell lysate

Ссылки

Даташит

Anti-NME6 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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