Anti-NOD1 Polyclonal Antibody (HV077014)

Name: Anti-NOD1 Polyclonal Antibody
Brand: abinScience
SKU: HV077014
Price: 168 USD
Availability: InStock

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Aperçu

Numéro de catalogue

HV077014

Description

Anti-NOD1 Polyclonal Antibody (HV077014) is a rabbit polyclonal antibody detecting NOD1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human NOD1 (Ala612-Asp826).

Cible

NOD1, Nucleotide-binding oligomerization domain-containing protein 1, Caspase recruitment domain-containing protein 4, CARD4

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9Y239

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Nucleotide-binding oligomerization domain-containing protein 1 (NOD1) is a ~107 kDa protein. Pattern recognition receptor (PRR) that detects bacterial peptidoglycan fragments and other danger signals and thus participates in both innate and adaptive immune responses. Specifically recognizes and binds gamma-D-glutamyl-meso-diaminopimelic acid (iE-DAP), a dipeptide present in peptidoglycan of Gram-negative bacteria. Preferentially binds iE-DAP in tripeptide-containing muropeptides (MurNAc-TriDAP or TriDAP). Ligand binding triggers oligomerization that facilitates the binding and subsequent activation of the proximal adapter receptor-interacting RIPK2. Following recruitment, RIPK2 undergoes 'Met-1'- (linear) and 'Lys-63'-linked polyubiquitination by E3 ubiquitin-protein ligases XIAP, BIRC2, BIRC3 and the LUBAC complex, becoming a scaffolding protein for downstream effectors, triggering activation of the NF-kappa-B and MAP kinases signaling.

1. Inohara, N. et al. (2001) The Journal of biological chemistry 276, 2551-4. PMID: 11058605

2. Girardin, SE. et al. (2003) Science (New York, N.Y.) 300, 1584-7. PMID: 12791997

3. Chamaillard, M. et al. (2003) Nature immunology 4, 702-7. PMID: 12796777

4. Tanabe, T. et al. (2004) The EMBO journal 23, 1587-97. PMID: 15044951

5. Girardin, SE. et al. (2005) The Journal of biological chemistry 280, 38648-56. PMID: 16172124

6. Fukazawa, A. et al. (2008) PLoS pathogens 4, e1000228. PMID: 19043560

7. Lautz, K. et al. (2012) Cellular microbiology 14, 1568-83. PMID: 22672233

8. Vegna, S. et al. (2016) Journal of virology 90, 6022-6035. PMID: 27099311

10. Magalhaes, JG. et al. (2005) EMBO reports 6, 1201-7. PMID: 16211083

Note

For research use only.

Images

Reférences

Fiche technique

Anti-NOD1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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