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Anti-NOP2 Polyclonal Antibody (HB503014)

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Vista general
Número de catálogoHB503014
Descripción
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant human NOP2 (Glu217-Ser588).
Objetivo Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase; 2.1.1.-; Nucleolar protein 1; Nucleolar protein 2 homolog; Proliferating-cell nucleolar antigen p120; Proliferation-associated nucleolar protein p120; NOP2; NOL1; NSUN1
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P46087, Q922K7
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

28S rRNA (cytosine(4447)-C(5))-methyltransferase (NOP2) is a ~89 kDa protein. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA. Required for efficient rRNA processing and 60S ribosomal subunit biogenesis. Regulates pre-rRNA processing through non-catalytic complex formation with box C/D snoRNAs and facilitates the recruitment of U3 and U8 snoRNAs to pre-90S ribosomal particles and their stable assembly into snoRNP complexes. May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation.

1. Bourgeois, G. et al. (2015) PloS one 10, e0133321. PMID: 26196125
2. Sloan, KE. et al. (2013) Cell reports 5, 237-47. PMID: 24120868
3. Liao, H. et al. (2022) Nucleic acids research 50, 10695-10716. PMID: 36161484
Nota For research use only
Imágenes
Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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