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Anti-NOSIP Polyclonal Antibody (HV105014)

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Vista general
Número de catálogoHV105014
Descripción
Anti-NOSIP Polyclonal Antibody (HV105014) is a rabbit polyclonal antibody detecting NOSIP in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Macaca fascicularis.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human NOSIP (Gly62-Ser295).
Objetivo eNOS-interacting protein, NOSIP, RING-type E3 ubiquitin transferase NOSIP, Nitric oxide synthase-interacting protein, E3 ubiquitin-protein ligase NOSIP
Purificación Purified by antigen affinity column.
Número de acceso Q9Y314
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Nitric oxide synthase-interacting protein (NOSIP) is a ~33 kDa protein. E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB. Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity.

1. Dedio, J. et al. (2001) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 15, 79-89. PMID: 11149895
2. Dreyer, J. et al. (2004) The Journal of neuroscience : the official journal of the Society for Neuroscience 24, 10454-65. PMID: 15548660
3. Schleicher, M. et al. (2005) Molecular and cellular biology 25, 8251-8. PMID: 16135813
Nota For research use only.
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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