Anti-NQO1 Polyclonal Antibody (HB232014)

Name: Anti-NQO1 Polyclonal Antibody
Brand: abinScience
SKU: HB232014
Price: 96 USD
Availability: InStock

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20ug
50ug
100ug

Número:

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Vista general

Número de catálogo

HB232014

Descripción

Anti-NQO1 Polyclonal Antibody (HB232014) is a rabbit polyclonal antibody detecting NQO1 in ELISA, IHC, WB. Suitable for Human, Mouse, Pongo abelii, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human NQO1 (Met1-Lys274).

Objetivo

NAD(P)H:quinone oxidoreductase 1, Azoreductase, Phylloquinone reductase, NQO1, DT-diaphorase, NAD(P)H dehydrogenase [quinone] 1, QR1, DIA4, NMOR1, Quinone reductase 1, Menadione reductase, DTD

Purificación

Purified by antigen affinity column.

Número de acceso

P15559

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

NAD(P)H dehydrogenase [quinone] 1 (NQO1) is a ~30 kDa protein. Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species. Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion.

1. Chen, S. et al. (1997) The Journal of biological chemistry 272, 1437-9. PMID: 8999809

2. Siegel, D. et al. (1997) Molecular pharmacology 52, 300-5. PMID: 9271353

3. Siegel, D. et al. (2004) Molecular pharmacology 65, 1238-47. PMID: 15102952

4. Asher, G. et al. (2005) Genes & development 19, 316-21. PMID: 15687255

5. Medina-Carmona, E. et al. (2017) Scientific reports 7, 44532. PMID: 28291250

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-NQO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Lncap cell lystae
Lane 2: LO2 cell lysate
Lane 3: A549 cell lysate

Referencias

Datasheet

Anti-NQO1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-NQO1 Polyclonal Antibody (HB232014)

Vista general

Imágenes

Referencias

Datasheet

SDS