Anti-NQO1 Polyclonal Antibody (HB232014)

Name: Anti-NQO1 Polyclonal Antibody
Brand: abinScience
SKU: HB232014
Price: 96 USD
Availability: InStock

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20ug
50ug
100ug

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SDS

Aperçu

Numéro de catalogue

HB232014

Description

Anti-NQO1 Polyclonal Antibody (HB232014) is a rabbit polyclonal antibody detecting NQO1 in ELISA, IHC, WB. Suitable for Human, Mouse, Pongo abelii, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human NQO1 (Met1-Lys274).

Cible

NAD(P)H:quinone oxidoreductase 1, Azoreductase, Phylloquinone reductase, NQO1, DT-diaphorase, NAD(P)H dehydrogenase [quinone] 1, QR1, DIA4, NMOR1, Quinone reductase 1, Menadione reductase, DTD

Purification

Purified by antigen affinity column.

Numéro d'accès

P15559

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

NAD(P)H dehydrogenase [quinone] 1 (NQO1) is a ~30 kDa protein. Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species. Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion.

1. Chen, S. et al. (1997) The Journal of biological chemistry 272, 1437-9. PMID: 8999809

2. Siegel, D. et al. (1997) Molecular pharmacology 52, 300-5. PMID: 9271353

3. Siegel, D. et al. (2004) Molecular pharmacology 65, 1238-47. PMID: 15102952

4. Asher, G. et al. (2005) Genes & development 19, 316-21. PMID: 15687255

5. Medina-Carmona, E. et al. (2017) Scientific reports 7, 44532. PMID: 28291250

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-NQO1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Lncap cell lystae
Lane 2: LO2 cell lysate
Lane 3: A549 cell lysate

Reférences

Fiche technique

Anti-NQO1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-NQO1 Polyclonal Antibody (HB232014)

Aperçu

Images

Reférences

Fiche technique

SDS