Anti-NR1H4/FXR Polyclonal Antibody (HV836014)

Name: Anti-NR1H4/FXR Polyclonal Antibody
Brand: abinScience
SKU: HV836014
Price: 168 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HV836014

説明

Anti-NR1H4/FXR Polyclonal Antibody (HV836014) is a rabbit polyclonal antibody detecting NR1H4 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human NR1H4/FXR (Leu211-Gln486).

ターゲット

Farnesoid X-activated receptor, Farnesol receptor HRR-1, HRR1, RXR-interacting protein 14, BAR, FXR, Bile acid receptor, NR1H4, RIP14, Nuclear receptor subfamily 1 group H member 4, Retinoid X receptor-interacting protein 14

精製

Purified by antigen affinity column.

アクセッション番号

Q96RI1

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Bile acid receptor (NR1H4/FXR) is a ~55 kDa protein. Ligand-activated transcription factor. Receptor for bile acids (BAs) such as chenodeoxycholic acid (CDCA), lithocholic acid, deoxycholic acid (DCA) and allocholic acid (ACA). Plays a essential role in BA homeostasis through the regulation of genes involved in BA synthesis, conjugation and enterohepatic circulation. Also regulates lipid and glucose homeostasis and is involved innate immune response. The FXR-RXR heterodimer binds predominantly to farnesoid X receptor response elements (FXREs) containing two inverted repeats of the consensus sequence 5'-AGGTCA-3' in which the monomers are spaced by 1 nucleotide (IR-1) but also to tandem repeat DR1 sites with lower affinity, and can be activated by either FXR or RXR-specific ligands.

1. Makishima, M. et al. (1999) Science (New York, N.Y.) 284, 1362-5. PMID: 10334992

2. Parks, DJ. et al. (1999) Science (New York, N.Y.) 284, 1365-8. PMID: 10334993

3. Modica, S. et al. (2010) Nuclear receptor signaling 8, e005. PMID: 21383957

4. Hollman, DA. et al. (2012) Biochimica et biophysica acta 1821, 1443-52. PMID: 22820415

5. Pircher, PC. et al. (2003) The Journal of biological chemistry 278, 27703-11. PMID: 12754200

6. Ananthanarayanan, M. et al. (2004) The Journal of biological chemistry 279, 54348-57. PMID: 15471871

7. Sanyal, S. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 15665-70. PMID: 17895379

8. Grober, J. et al. (1999) The Journal of biological chemistry 274, 29749-54. PMID: 10514450

9. Neimark, E. et al. (2004) Hepatology (Baltimore, Md.) 40, 149-56. PMID: 15239098

10. Landrier, JF. et al. (2006) American journal of physiology. Gastrointestinal and liver physiology 290, G476-85. PMID: 16269519

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-NR1H4/FXR Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

抗体

アッセイキット

Anti-NR1H4/FXR Polyclonal Antibody (HV836014)

概要

イメージ

参考文献

データシート

SDS