Anti-NTMT2 Polyclonal Antibody (HB306014)

Name: Anti-NTMT2 Polyclonal Antibody
Brand: abinScience
SKU: HB306014
Price: 96 USD
Availability: InStock

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Especificación:

20ug
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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HB306014

Descripción

Anti-NTMT2 Polyclonal Antibody (HB306014) is a rabbit polyclonal antibody detecting N-terminal Xaa-Pro-Lys N-methyltransferase 2; 2.1.1.299; Alpha N-terminal protein methyltransferase 1B; Methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B; NTM1B; NTMT2; C1orf184; METTL11B; NRMT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant human NTMT2 (Met1-Ser283).

Objetivo

N-terminal Xaa-Pro-Lys N-methyltransferase 2; 2.1.1.299; Alpha N-terminal protein methyltransferase 1B; Methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B; NTM1B; NTMT2; C1orf184; METTL11B; NRMT2

Nivel de endotoxinas

Please contact with the lab for this information.

Purificación

Purified by antigen affinity column.

Número de acceso

Q5VVY1, B2RXM4, D3ZVR1

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

N-terminal Xaa-Pro-Lys N-methyltransferase 2 (NTMT2) is a ~32 kDa protein. Alpha N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes monomethylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and Pro in the Pro-Pro-Lys motif. Predominantly functions as a mono-methyltransferase but is also able to di-/tri-methylate the GPKRIA peptide and di-methylate the PPKRIA peptide (in vitro). May activate NTMT1 by priming its substrates for trimethylation.

1. Petkowski, JJ. et al. (2013) The Biochemical journal 456, 453-62. PMID: 24090352

2. Dong, C. et al. (2018) Communications biology 1, 183. PMID: 30417120

Nota

For research use only

Imágenes

Western blot

Western blot analysis was performed using anti-NTMT2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse heart lysate
Lane 2: Rat heart lysate

Referencias

Datasheet

Anti-NTMT2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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