Anti-NTMT2 Polyclonal Antibody (HB306014)

Name: Anti-NTMT2 Polyclonal Antibody
Brand: abinScience
SKU: HB306014
Price: 96 USD
Availability: InStock

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Aperçu
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HB306014

Description

Anti-NTMT2 Polyclonal Antibody (HB306014) is a rabbit polyclonal antibody detecting N-terminal Xaa-Pro-Lys N-methyltransferase 2; 2.1.1.299; Alpha N-terminal protein methyltransferase 1B; Methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B; NTM1B; NTMT2; C1orf184; METTL11B; NRMT2 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant human NTMT2 (Met1-Ser283).

Cible

N-terminal Xaa-Pro-Lys N-methyltransferase 2; 2.1.1.299; Alpha N-terminal protein methyltransferase 1B; Methyltransferase-like protein 11B; X-Pro-Lys N-terminal protein methyltransferase 1B; NTM1B; NTMT2; C1orf184; METTL11B; NRMT2

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q5VVY1, B2RXM4, D3ZVR1

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

N-terminal Xaa-Pro-Lys N-methyltransferase 2 (NTMT2) is a ~32 kDa protein. Alpha N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes monomethylation of exposed alpha-amino group of Ala or Ser residue in the [Ala/Ser]-Pro-Lys motif and Pro in the Pro-Pro-Lys motif. Predominantly functions as a mono-methyltransferase but is also able to di-/tri-methylate the GPKRIA peptide and di-methylate the PPKRIA peptide (in vitro). May activate NTMT1 by priming its substrates for trimethylation.

1. Petkowski, JJ. et al. (2013) The Biochemical journal 456, 453-62. PMID: 24090352

2. Dong, C. et al. (2018) Communications biology 1, 183. PMID: 30417120

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-NTMT2 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse heart lysate
Lane 2: Rat heart lysate

Reférences

Fiche technique

Anti-NTMT2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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