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Anti-Nucleoprotein Polyclonal Antibody (VK432024)

Anti-Nucleoprotein Polyclonal Antibody
Anti-Nucleoprotein Polyclonal Antibody
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概要
カタログ番号VK432024
説明
Anti-Nucleoprotein Polyclonal Antibody (VK432024) is a rabbit polyclonal antibody detecting Nucleoprotein in ELISA, IHC, WB. Suitable for Hendra virus and Nipah virus.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
種反応性Hendra virus (HeV), Nipah virus (NiV)
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant hendra virus Nucleoprotein (Met1-Ala394).
ターゲット nucleocapsid, N
エンドトキシンレベル Please contact with the lab for this information.
精製 Purified by antigen affinity column.
アクセッション番号 Q9IK92, O89339
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Nucleoprotein is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 13.4 N per turn, approximately 20 nm in diameter, with a hollow central cavity of 56 nm in diameter. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P.

1. Ker, DS. et al. (2021) PLoS pathogens 17, e1009740. PMID: 34270629
2. Yabukarski, F. et al. (2014) Nature structural & molecular biology 21, 754-9. PMID: 25108352
注意事項 For research use only
イメージ
  • Anti-Nucleoprotein Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-Nucleocapsid-Hendra virus polyclonal antibody at 1ug/mL on various samples.
    Lane 1: ATG18514 100ng

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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