Anti-NUP85 Polyclonal Antibody (HV235014)

Name: Anti-NUP85 Polyclonal Antibody
Brand: abinScience
SKU: HV235014
Price: 96 USD
Availability: InStock

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Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HV235014

Descripción

Anti-NUP85 Polyclonal Antibody (HV235014) is a rabbit polyclonal antibody detecting NUP85 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human NUP85 (Arg130-Leu368).

Objetivo

Pericentrin-1, Nucleoporin Nup85, Nucleoporin Nup75, PCNT1, 85 kDa nucleoporin, NUP75, NUP85, Nuclear pore complex protein Nup85, FROUNT

Purificación

Purified by antigen affinity column.

Número de acceso

Q9BW27

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Nuclear pore complex protein Nup85 is a ~75 kDa protein. Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP96/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2-activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl-inositol 3-kinase-Rac-lammellipodium protrusion cascade.

1. Harel, A. et al. (2003) Molecular cell 11, 853-64. PMID: 12718872

2. Orjalo, AV. et al. (2006) Molecular biology of the cell 17, 3806-18. PMID: 16807356

3. Terashima, Y. et al. (2005) Nature immunology 6, 827-35. PMID: 15995708

4. Braun, DA. et al. (2018) The Journal of clinical investigation 128, 4313-4328. PMID: 30179222

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-NUP85 polyclonal antibody at 1μg/ml on various samples.
Lane 1: Hela cell lysate
Lane 2: HT-29 cell lysate
Lane 3: LO2 cell lysate
Lane 4: 293T cell lysate

Referencias

Datasheet

Anti-NUP85 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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