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Anti-OGA Polyclonal Antibody (HT134014)

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Aperçu
Numéro de catalogueHT134014
Description
Anti-OGA Polyclonal Antibody (HT134014) is a rabbit polyclonal antibody detecting OGA in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human OGA (Ser6-Lys240).
Cible OGA, Protein O-GlcNAcase, Meningioma-expressed antigen 5, Beta-N-acetylhexosaminidase, HEXC, N-acetyl-beta-glucosaminidase, KIAA0679, Nuclear cytoplasmic O-GlcNAcase and acetyltransferase, MEA5, MGEA5, Beta-hexosaminidase, NCOAT, Beta-N-acetylglucosaminidase, N-acetyl-beta-D-glucosaminidase
Purification Purified by antigen affinity column.
Numéro d'accès O60502
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Protein O-GlcNAcase (OGA) is a ~102 kDa protein. Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.

1. Gao, Y. et al. (2001) The Journal of biological chemistry 276, 9838-45. PMID: 11148210
2. Wells, L. et al. (2002) The Journal of biological chemistry 277, 1755-61. PMID: 11788610
3. Li, J. et al. (2010) Biochemistry. Biokhimiia 75, 938-43. PMID: 20673219
4. Schimpl, M. et al. (2012) Chemistry & biology 19, 173-8. PMID: 22365600
5. Rao, FV. et al. (2013) Open biology 3, 130021. PMID: 24088714
6. Li, B. et al. (2017) Nature communications 8, 666. PMID: 28939839
7. Yu, F. et al. (2024) Inflammation research : official journal of the European Histamine Research Society ... [et al.] 73, 5-17. PMID: 37962578
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-OGA Polyclonal Antibody

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