Anti-ORF8/ns8 Polyclonal Antibody (VK460014)

Name: Anti-ORF8/ns8 Polyclonal Antibody
Brand: abinScience
SKU: VK460014
Price: 168 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

VK460014

Description

Anti-ORF8/ns8 Polyclonal Antibody (VK460014) is a rabbit polyclonal antibody detecting ORF8 protein in ELISA, IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant SARS-CoV-2 ORF8/ns8 (Phe16-Ile121).

Cible

ORF8 protein, ORF8, Non-structural protein 8, ns8

Purification

Purified by antigen affinity column.

Numéro d'accès

P0DTC8

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

ORF8 protein (ns8) is a ~13 kDa protein. Plays a role in modulating the host immune response. May act as a secreted virokine by mimicking interleukin-17A (IL17A), and thereby binding to the IL17RA receptor, leading to activation of the IL17 pathway and increased secretion of pro-inflammatory factors. Contributes to the cytokine storm during SARS-CoV-2 infection when secreted by unconventional pathway. May act by down-regulating major histocompability complex class I (MHC-I) at cell surface. May inhibit expression of some members of the IFN-stimulated gene (ISG) family including hosts IGF2BP1/ZBP1, MX1 and MX2, and DHX58.

1. Chan, JF. et al. (2020) Lancet (London, England) 395, 514-523. PMID: 31986261

2. Wu, X. et al. (2022) mBio 13, e0040222. PMID: 35343786

3. Lin, X. et al. (2023) PLoS pathogens 19, e1011128. PMID: 36689483

4. Lin, X. et al. (2021) iScience 24, 102293. PMID: 33723527

5. Zhang, Y. et al. (2021) Proceedings of the National Academy of Sciences of the United States of America 118. PMID: 34021074

6. Matsuoka, K. et al. (2022) The Journal of biological chemistry 298, 101724. PMID: 35157849

7. Geng, H. et al. (2021) Frontiers in immunology 12, 679482. PMID: 34177923

Note

For research use only.

Images

Reférences

Fiche technique

Anti-ORF8/ns8 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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