Anti-PAK1 Polyclonal Antibody (HC494014)

Name: Anti-PAK1 Polyclonal Antibody
Brand: abinScience
SKU: HC494014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Datasheet
SDS

Vista general

Número de catálogo

HC494014

Descripción

Anti-PAK1 Polyclonal Antibody (HC494014) is a rabbit polyclonal antibody detecting PAK1 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pan troglodytes, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human PAK1 (Glu252-Cys501).

Objetivo

Alpha-PAK, p65-PAK, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, PAK1

Purificación

Purified by antigen affinity column.

Número de acceso

Q13153

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Serine/threonine-protein kinase PAK 1 (PAK1) is a ~60 kDa protein. Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as a GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases.

1. Zenke, FT. et al. (1999) The Journal of biological chemistry 274, 32565-73. PMID: 10551809

2. Manabe, R. et al. (2002) Journal of cell science 115, 1497-510. PMID: 11896197

3. Slack-Davis, JK. et al. (2003) The Journal of cell biology 162, 281-91. PMID: 12876277

4. Zhou, GL. et al. (2003) Molecular and cellular biology 23, 8058-69. PMID: 14585966

5. Zhou, H. et al. (2005) The Journal of biological chemistry 280, 10624-35. PMID: 15611088

6. Rider, L. et al. (2007) The Journal of biological chemistry 282, 30985-96. PMID: 17726028

7. Mayhew, MW. et al. (2007) Journal of cell science 120, 3911-8. PMID: 17989089

8. Harms, FL. et al. (2018) American journal of human genetics 103, 579-591. PMID: 30290153

9. Acconcia, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6782-7. PMID: 17420447

10. Brown, JL. et al. (1996) Current biology : CB 6, 598-605. PMID: 8805275

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-PAK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo