Anti-PAK1 Polyclonal Antibody (HC494014)

Name: Anti-PAK1 Polyclonal Antibody
Brand: abinScience
SKU: HC494014
Price: 168 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HC494014

説明

Anti-PAK1 Polyclonal Antibody (HC494014) is a rabbit polyclonal antibody detecting PAK1 in ELISA, IHC, WB. Suitable for Bovine, Human, Mouse, Pan troglodytes, and Pongo pygmaeus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

種反応性

Human, Mouse, Rat

アプリケーション

ELISA, IHC, WB

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

免疫原

E. coli - derived recombinant Human PAK1 (Glu252-Cys501).

ターゲット

Alpha-PAK, p65-PAK, PAK-1, Serine/threonine-protein kinase PAK 1, p21-activated kinase 1, PAK1

精製

Purified by antigen affinity column.

アクセッション番号

Q13153

形態

Liquid

保存バッファー

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Serine/threonine-protein kinase PAK 1 (PAK1) is a ~60 kDa protein. Protein kinase involved in intracellular signaling pathways downstream of integrins and receptor-type kinases that plays an important role in cytoskeleton dynamics, in cell adhesion, migration, proliferation, apoptosis, mitosis, and in vesicle-mediated transport processes. Can directly phosphorylate BAD and protects cells against apoptosis. Activated by interaction with CDC42 and RAC1. Functions as a GTPase effector that links the Rho-related GTPases CDC42 and RAC1 to the JNK MAP kinase pathway. Phosphorylates and activates MAP2K1, and thereby mediates activation of downstream MAP kinases.

1. Zenke, FT. et al. (1999) The Journal of biological chemistry 274, 32565-73. PMID: 10551809

2. Manabe, R. et al. (2002) Journal of cell science 115, 1497-510. PMID: 11896197

3. Slack-Davis, JK. et al. (2003) The Journal of cell biology 162, 281-91. PMID: 12876277

4. Zhou, GL. et al. (2003) Molecular and cellular biology 23, 8058-69. PMID: 14585966

5. Zhou, H. et al. (2005) The Journal of biological chemistry 280, 10624-35. PMID: 15611088

6. Rider, L. et al. (2007) The Journal of biological chemistry 282, 30985-96. PMID: 17726028

7. Mayhew, MW. et al. (2007) Journal of cell science 120, 3911-8. PMID: 17989089

8. Harms, FL. et al. (2018) American journal of human genetics 103, 579-591. PMID: 30290153

9. Acconcia, F. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 6782-7. PMID: 17420447

10. Brown, JL. et al. (1996) Current biology : CB 6, 598-605. PMID: 8805275

注意事項

For research use only.

イメージ

参考文献

データシート

Anti-PAK1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

組換えタンパク質

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アッセイキット

Anti-PAK1 Polyclonal Antibody (HC494014)

概要

イメージ

参考文献

データシート

SDS

クイックアクセス

お問い合わせ