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Anti-PAM Polyclonal Antibody (HB756014)

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Aperçu
Numéro de catalogueHB756014
Description
Anti-PAM Polyclonal Antibody (HB756014) is a rabbit polyclonal antibody detecting PAM in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactivité des espècesHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human PAM (Phe21-Cys288).
Cible PAL, PHM, PAM, Peptidylamidoglycolate lyase, Peptidyl-glycine alpha-amidating monooxygenase
Purification Purified by antigen affinity column.
Numéro d'accès P19021
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Peptidyl-glycine alpha-amidating monooxygenase (PAM) is a ~108 kDa protein. Bifunctional enzyme that catalyzes amidation of the C-terminus of proteins. Alpha-amidation is present at the C-terminus of many endocrine hormones and neuropeptides and is required for their activity. C-terminal amidation also takes place in response to protein fragmentation triggered by oxidative stress, promoting degradation of amidated protein fragments by the proteasome. Alpha-amidation involves two sequential reactions, both of which are catalyzed by separate catalytic domains of the enzyme. The first step, catalyzed by peptidyl alpha-hydroxylating monooxygenase (PHM) domain, is the copper-, ascorbate-, and O2- dependent stereospecific hydroxylation (with S stereochemistry) at the alpha-carbon (C-alpha) of the C-terminal glycine of the peptidylglycine substrate.

1. Satani, M. et al. (2003) Protein expression and purification 28, 293-302. PMID: 12699694
2. Glauder, J. et al. (1990) Biochemical and biophysical research communications 169, 551-8. PMID: 2357221
3. Eipper, BA. et al. (1992) Annual review of neuroscience 15, 57-85. PMID: 1575450
4. Stadtman, ER. (1990) Biochemistry 29, 6323-31. PMID: 2207077
Note For research use only.
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-PAM Polyclonal Antibody

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