Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application
Dilution
ELISA
1:5000-1:20000
IHC
1:50-1:500
WB
1:500-1:2000
Stabilité et stockage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte
RAC-alpha serine/threonine-protein kinase (Pan-AKT,AKT1,AKT2,AKT3) is a ~55 kDa protein. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling.
1. Nicholson, KM. et al. (2002) Cellular signalling 14, 381-95. PMID: 11882383
2. Rönnstrand, L. (2004) Cellular and molecular life sciences : CMLS 61, 2535-48. PMID: 15526160
3. Cartlidge, RA. et al. (2005) The EMBO journal 24, 1696-705. PMID: 15861136
4. Heron-Milhavet, L. et al. (2011) Histology and histopathology 26, 651-62. PMID: 21432781
5. Hers, I. et al. (2011) Cellular signalling 23, 1515-27. PMID: 21620960
6. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173
7. Choppara, S. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 998-1003. PMID: 29343641
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