Anti-PARN Polyclonal Antibody (HF535014)

Name: Anti-PARN Polyclonal Antibody
Brand: abinScience
SKU: HF535014
Price: 168 USD
Availability: InStock

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Каталожный номер

HF535014

Описание

Anti-PARN Polyclonal Antibody (HF535014) is a rabbit polyclonal antibody detecting PARN in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human PARN (Lys178-Asp245).

Цель

Deadenylating nuclease, Polyadenylate-specific ribonuclease, Poly(A)-specific ribonuclease PARN, Deadenylation nuclease, PARN, DAN

Очистка

Purified by antigen affinity column.

Номер доступа

O95453

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Poly(A)-specific ribonuclease PARN is a ~73 kDa protein. 3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP.

1. Gao, M. et al. (2000) Molecular cell 5, 479-88. PMID: 10882133

2. Martînez, J. et al. (2001) The Journal of biological chemistry 276, 27923-9. PMID: 11359775

3. Lai, WS. et al. (2003) Molecular and cellular biology 23, 3798-812. PMID: 12748283

4. Gherzi, R. et al. (2004) Molecular cell 14, 571-83. PMID: 15175153

5. Körner, CG. et al. (1998) The EMBO journal 17, 5427-37. PMID: 9736620

6. Berndt, H. et al. (2012) RNA (New York, N.Y.) 18, 958-72. PMID: 22442037

7. Boele, J. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 11467-72. PMID: 25049417

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-PARN Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

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Anti-PARN Polyclonal Antibody (HF535014)

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